Method of analysis of Sildenafil citrate Eq. to sildenafil citrate Tablets (Assay)

Test arrangement: Weigh and powder 20 tablets. Scatter an amount of powder containing around 50 mg of Sildenafil with 35 ml of dissolvable blend, sonicate for 20 minutes and weaken to 50.0 ml with the dissolvable blend, channel. Further weaken 5 ml to 50 ml with dissolvable blend.
Reference solution:(Standard arrangement) : A 0.14 percent w/v arrangement of Sildenafil citrate RS in the dissolvable blend. Further weaken 5 ml to 50 ml with dissolvable blend to peptides bodybuilding.
Chromatographic framework
- Column : a tempered steel section 25 cm x 4.6 mm, pressed with silica (5 µm) (Such as Zobrax Eclipse XBD C18),
- Mobile stage: a blend of 30 volumes of arrangement containing 3.85 g of ammonium acetic acid derivation and 1.0 ml of triethylamine in 1000 ml of water, change in accordance with pH 5.5 with acidic corrosive and 70 volumes of methanol,
- Flow rate: 1 ml for each moment to peptides bodybuilding.